Dr
PATAI
CHAROONNART
Educations
2002-2006: B.Sc. Genetics, Department of Botany, Faculty of Science, Chulalongkorn University
2006-2008: M.Sc. Biotechnology (Botany), Faculty of Science, Chulalongkorn University
2009-2015: Ph.D. Biological Science (Botany), Faculty of Science, Chulalongkorn University
Job Appointments
2018-present Postdoctoral researcher, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Mahidol University, Thailand
2016-2018 Postdoctoral researcher, National Center for Genetic Engineering and Biotechnology (BIOTEC), Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Mahidol University, Thailand
2008-2009 Research assistance, Center for shrimp molecular biology and biotechnology, Faculty of Science, Mahidol University
2004 (3 months) Internship, Central institute of forensic science Thailand, Ministry of Justice, Thailand
On-going research
Use of Chlamydomonas reinhardtii to produce double stranded RNA for shrimp virus protection
Achievements
Publications:
Charoonnart, P., Worakajit, N., Meetam, M., Robinson, C. & Saksmerprome, V. Light-driven production of microalga C. reinhardtii expressing dsRNA against shrimp virus. (In preparation) Charoonnart, P., Purton, S., & Saksmerprome, V. (2018). Applications of Microalgal Biotechnology for Disease Control in Aquaculture. Biology, 7(2), 24. Thammasorn T, Jitrakorn S, Charoonnart P, Sirimanakul S, Rattanarojpong T, Chaturongakul S, Saksmerprome V (2017) “Probiotic bacteria (Lactobacillus plantarum) expressing specific double-stranded RNA and its potential for controlling shrimp viral and bacterial diseases” Aquaculture International 25, 1679-1692 Saksmerprome V., Charoonnart P, Flegel, T.W. (2017) “Feasibility of dsRNA treatment for post-clearing SPF shrimp stocks of newly discovered viral infections using Laem Singh virus (LSNV) as a model” Virus Research, 235, 73-76 Charoonnart, P., Seraypheap, K., Chadchawan, S., & Wangsomboondee, T. (2016). Arbuscular mycorrhizal fungus improves the yield and quality of Lactuca sativa in an organic farming system. SCIENCEASIA, 42(5), 315-322. Charoonnart, P., Seraypheap, K., Chadchawan, S., and Wangsomboondee, T. 2015. Growth improvement of butterhead lettuce by Glomus mosseae in field cultivation. Thailand Journal of Botany. 7: 173-181. Charoonnart, P., Seraypheap, K., Chadchawan, S., & Wangsomboondee, T. (2016). Increasing butterhead lettuce yield using organic methods and application of arbuscular mycorrhizal fungi. International Journal of Vegetable Science, 22(6), 520-529.Saksmerprome, V., Charoonnart, P., Gangnonngiw, W., and Withyachumnarnkul, B. (2009) “A novel and inexpensive application of RNAi technology to protect shrimp from viral disease.” J Virol Methods, 162:213-217
Expertises Plant physiology; plantmicrobial interaction, postharvest physiology, plant stress responses Microalgal biotechnology; chloroplast engineering RNA interference Molecular techniques; nucleic acid based techniques Phytochemistry; plant enzymes assayDr. Patai Charoonnart got her Ph.d. in Botany from program of Biological Science, Chulalongkorn University in 2016. After that, she has started her work as postdoctoral researcher in Shrimp Molecular Biology and Biotechnology laboratory, National Center for Genetic Engineering and Biotechnology, Thailand. Presently, she is postdoctoral researcher at Center of Excellence for Shrimp Molecular Biology and Biotechnology, faculty of Science, Mahidol University. Her work is focusing on production and delivery of dsRNA for controlling shrimp and fish viral diseases. Currently, with her interest, she is exploring application of microalgal chloroplast engineering for being used as a tool to improve and support aquaculture quality, mainly on remedying loss by viral diseases.
As mentioned above, my focus is the use of microalgal biotechnology as a tool for producing antimicrobial biomolecules. It is because microalgae can be an effective bio-carrier for delivering those biomolecules into animal through oral administration. Currently, I have constructed microalgae to produce dsRNA targeting virus in shrimp. We are also would like to expand our work to produce dsRNA targeting fish virus as well as subunit vaccines or proteins involving shrimp and fish immune boosting. Another target protein that I am interested is an endolysin which specific to bacterial pathogen.