I am vinita sharma just completed masters in biochemistry from central university of Rajasthan, India. I have explored the field of vaccinology in infectious diseases, now want to explore the veterinary vaccinology.

Academics

Integrated MSc in Biochemistry (2015-2020), CentralUniversity of Rajasthan, Bandarsindri, Ajmer, Rajasthan (CGPA 8.23/10) Intermediate in-  Science Biology (2013-2014)-  Board of Secondary Education , Rajasthan (85.67%/100) 10th (2012) ) Board of Secondary Education , Rajasthan (79%/100)

Academic achievements/Awards

Gargi Puraskar awarded Indira Gandhi Priyadarshini Awarded INMIS-2019 travel award of 2500CDN to attend the 5th International Neonatal and Maternal Immunization Symposium, held in Vancouver Canada Summer Research Fellowship 2019 by the JNCASR to participate in the two month summer research program.

National Level Examination

     

Central University Common Entrance Test (CUCET) (May 2015), conducted by Central Universities  of India General Aptitude Test in Engineering (GATE) (Jan 2020), GATE score 418, conducted jointly by Indian Institute of Sciences (IISc) and seven Indian Institute of Technology

 

Participated conference/symposium

Participated in International Conference on Molecular Basis of Diseases and Therapeutics (ICMBDT -2019). Participated in Poster Presentation and Secured Second Position At the Departmental Research Activity -2018 Organized by Department of Biochemistry, School of Life Sciences, and Central University of Rajasthan. Participated in Poster Presentation and  First (Innovative Proposal)  Position At the Departmental Research Activity -2019Organized by Department of Biochemistry, School of Life Sciences, Central University Of  Rajasthan

Membership with international networks

Life-member- HIC-Vac network, Paddington, London, United Kingdom Life-member- International Society for Vaccines (ISV)

 

Project Summary

                  I have completed my M.Sc. dissertation on the project entitled “Immunoglobulin Interface Redesigning to Enhance Lebrikizumab Mediated Immunomodulation of IL-13 Hyper-response” under the supervision of Dr. Vijay Kumar Prajapati assistant professor, laboratory of infectious diseases, in the Department of Biochemistry at Central University of Rajasthan, India. In this project we have to tried to find out some advantageous mutations which can increase the affinity of the antibody against their particular target. Also the work has been done on how diagnostic antibodies are used as therapeutic antibodies by SDR grafting to human antibodies (Humanization). I offer a summary of my undergraduate degree thesis, it was my first extended research experience under the guidance of same (above). The project was focused on the designing of a multi-epitope subunit vaccine against Epstein-Barr virus-mediated malignancy. I pioneered most of the computational techniques of vaccine designing. I also want to emphasise that my interest of research in the field of diabetes, a project under the supervision of Prof. M. Balasubramanyam, Dean of Research Studies & Senior Scientist, Madras Diabetes Research Foundation, Chennai, India. My project was on the “Importance of Exosomes in Metabolic Disorder with special reference to Diabetes”. In which I was explored to the experiments that how healthy Exosomes are beneficial to the diabetic patients.

Techniques Expertise

RNA and DNA isolation, SDS PAGE, Western Blotting,  PCR,  Bacterial culture, Tissue Culture, Wound Healing Assay, Microscopy, Primer Designing, PBMC Isolation, Chromatography (Paper, Column), Spectrometry,  ELISA and other basic Immunological techniques, Enzymatic Assay and Basic rDNA techniques. Computational techniques of vaccine designing including epitope sorting, homology modelling, molecular docking (ClusPro, Zdock, Rosetta, and Glide) and Molecular Dynamic Simulation (Gromacs and Desmond).

Research Interest

The signalling of pathogenesis and immunological mechanism behind the infectious diseases. Vaccine development against infectious diseases. To investigate the signalling of different cellular proteins as utilize them as therapeutic targets.

Publications

Sharma V., Chauhan P., Pandey R.K., Ojha R., Aathmanathan V.S., Krishnan M., Prajapati V.K. (2020). Immunoglobulin interface redesigning to enhance Lebrikizumab mediated immunomodulation of IL-13 hyper-response. Journal of Biomolecular Structure and Dynamics. DOI: https://doi.org/10.1080/07391102.2020.1773316 (IF- 3.310)

 

Sharma V., Chauhan P., Pandey R.K., Ojha R., Aathmanathan V.S., Krishnan M., Prajapati V.K. (2020). Anti-IL-10 antibody humanization by SDR grafting with enhanced affinity to neutralize the adverse response of interleukin-10.  Journal of structural biology, 2020- Elsevier (Under communication)  Book-Chapter: Sharma, V., Pandey, R.K., Prajapati, V.K. (2020). Treatment strategies for colon cancer. (Under communication). Chauhan, P., Sharma, V., Pandey, R.K., Prajapati, V.K. (2020). Recent advances in the therapeutic development of prostate cancer. (Under communication)