Haroon Afzal

Mr
Haroon
Afzal

PhD Student
National Pingtung UNiversity of Science and Technology
Email 
haroonafzal963 [at] gmail.com
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Biography
PhD Scholar, International Degree Program in Animal Vaccine Technology, Taiwan 2021- ongoing Master in Animal Vaccine Technology, Taiwan 2019-2021 Doctor of Veterinary Medicine, University of Agriculture, Faisalabad 2013-2018
Research interests
Nucleic acid technology: DNA/RNA extraction, PCR, qPCR, cloning Protein techniques: Protein analysis, protein expression, SDS-PAGE, western blot, and protein purification. In vitro: Cell culture, Mice, chickens, ducks, porcine, goats, bovines, fish Animal vaccines and adjuvants: Inactivated, subunit, DNA vaccines, biological adjuvants (TLR2, TLR5), monoclonal antibody, IgY. Immune evaluations: ELSIA, flow-cytometry, T cell proliferation, HA, HI, neutralization.
Projects you're working on
Study truncated domains of flagellin as adjuvant Working of TLR2 agonist as adjuvant Lipidation of antigen to achieve TLR2 activation
Discipline
Immunology – B-cells Immunology – T-cells Immunology – innate Molecular biology Protein biology Structural biology Virology
Host species
Pigs Poultry
Pathogen
VirusesCoronavirus VirusesInfectious bursal disease virus
Stage of vaccine development
Adjuvants Antigen discovery and immunogen design Vaccine delivery