TECHNICAL SKILLS Wet Lab: • Developed and standardized IgM-capture ELISA for acute infection detection and IgG ELISA for identifying past infection or vaccine-induced antibodies. • Cell Culture & Transfection: Lipofectamine transfection, MTT cytotoxicity, hypermethylation studies • Immunoassays & Protein Techniques: Immunohistochemistry, hybridoma (monoclonal antibody production/purification), ELISPOT, ELISA (direct/indirect/competitive), lateral flow assays (LFIA), fluorescent polarization, spectrophotometry, immunoprecipitation, Western blot • Molecular Biology: Nucleic acid extraction/quantification, PCR, molecular cloning, recombinant antigen expression (prokaryotic and eukaryotic) • Diagnostics & Animal Work: Agglutination tests (RBPT, SAT, MAT), microscopy, hemagglutination/inhibition assays, vaccine testing (safety/sterility/potency), rodent inoculation, animal immunization • Additional: Protein crystallization, mass spectrometry, flow cytometry (basic FACS) Dry Lab & Data Analysis: • Bioinformatics & NGS: NGS data analysis (RNA-seq, variant calling, metagenomics, microarrays), Linux, cloud computing, Python • CADD & Statistics: Computer-aided drug design, R applications, machine learning, GraphPad, Origin, SPSS • Other Software: Microsoft Office, Image-J, QGIS Professional Responsibilities • Literature review and experimental design • Execution and analysis of experiments • Assisting in organization of national seminars/training programs • Requisition management and project meeting participation • Preparation of progress reports, manuscripts, presentations, annual reports • Project budget monitoring and team collaboration PROFESSIONAL EXPERIENCE 8+ years of research in molecular biology, diagnostics, and assay development (BSL2+ laboratories): • Research Scholar (2023–Present) ICAR-NIVEDI, Bengaluru (BSL2+ Lab) Project: Production & Characterization of Monoclonal Antibodies Against BTV Serotype • Young Professional-II (2023–2024) ICAR-NIVEDI, Bengaluru (BSL2+ Lab) Project: Characterization of bluetongue virus strains and vaccine candidate assessment • Research Associate-III (2021–2023) ICAR-NIVEDI, Bengaluru (BSL2+ Lab) Validation/Field Testing of DIVA tests for brucellosis surveillance (DBT-ADMaC Phase-II) • Junior Research Fellow (multiple projects, 2015–2021) ICAR-NIVEDI, NIMHANS, IVRI, NICPR – BSL2+ & Cancer/Viral/Parasitic Diagnostic Labs Projects in monoclonal antibody production, brucellosis, cysticercosis, IBD vaccine, HPV diagnostics, cancer therapeutics, and method development • Research Assistant & Trainee (2011–2014) National Institute of Cancer Prevention and Research (NICPR); Codon Biotech; IMS Engineering College Projects on HPV, plant antimicrobials, dengue serology, molecular docking, advanced biotechnology training EDUCATION 1. Master of Technology (M.Tech), Biotechnology Gold Medalist; 90.7%, IMS Engineering College, Ghaziabad (2012 – 2014) 2. Bachelor of Technology (B.Tech), Biotechnology Gold Medalist; 82.3%, IILM College of Engineering & Technology, Greater Noida (2008 – 2012) SELECTED PUBLICATIONS 1. Siju Susan Jacob, Pinaki Prasad Sengupta, S. Shamshad, et al. (2025) Development of an enzyme immunoassay employing low molecular weight recombinant Ag1 and Ag1V1 proteins for porcine cysticercosis. Vet Parasitol, 338:110532. 2. Sinha PR, Hegde SR, Mittal R, Jagat CC, Gowda U, Chandrashekhar R, Muthaiah G, Shamshad S, Chanda MM, Ganji V, Putty K, Hemadri D. (2024) In silico development of a multi-epitope subunit vaccine against Bluetongue virus in Ovis aries using immunoinformatic approach. Pathogens, 13(11):944. 3. Shome R, Patil S, Shamshad S, Skariah S, Sahib MM, Shanmugam G, Barman NN, Bora DP, Shome A. (2024) Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISA. Indian J Anim Sci, 94(12), 1022–1030. 4. Shome R, Patra S, Sahib MM, Shanmugam G, Skariah S, Shamshad S, et al. (2025) Chimeric protein A/G conjugate based lateral flow assay for brucellosis detection. J Immunol Methods, 518:113845. 5. Jacob SS, Sengupta PP, Chandu AGS, Shamshad S, et al. (2021) Existence of genetic lineages within Asian genotype of Taenia solium. Transboundary and Emerging Diseases, 69(4):2256-2265. 6. Rajeswari Shome, Muneera Mohamed Sahib, Sudipta Patra, G. Shanmugam, Shiva Dubey, Samer Shamshad, et al. (2023) Evaluation of an in-house IgM/IgG lateral flow assay for human brucellosis. Indian J Med Microbiol, 42(6):55–58. 7. Sudipta Patra, Muneera M. Sahib, G. Shanmugam, Somy Skariah, Samer Shamshad, et al. (2023) Evaluation of an In-house Indirect ELISA for IgM and IgG anti-Brucella Antibodies in Human Brucellosis. J Pure Appl Microbiol, 17(3):1650-1658. Full publication list is available on google scholar: https://scholar.google.com/citations?hl=en&user=AfHxvTAAAAAJ ACADEMIC AWARDS • Cracked All India Graduate Aptitude Test in Engineering (GATE-2022) organized by IIT-Kharagpur REFERENCES, EDUCATIONAL DOCUMENTS AND EXPERIENCE CERTIFICATES: Available on request.